PSMD10/Gankyrin Recombinant antibody

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  WB analysis using 86120-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86120-2-RR (PSMD10/Gankyrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 86120-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86120-2-RR (PSMD10/Gankyrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 86120-2-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86120-2-RR (PSMD10/Gankyrin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PSMD10/Gankyrin Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 86120-2-RR

Clone No.250170D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

PSMD10, 26S proteasome non-ATPase regulatory subunit 10, Gankyrin, p28, p28(GANK)

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Tested Applications

• Positive WB detected in: A549 cells, HEK-293 cells, HeLa cells, PC-3 cells, K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86120-2-RR targets PSMD10/Gankyrin in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PSMD10/Gankyrin fusion protein Ag2994
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
• Calculated Molecular Weight: 226 aa, 24 kDa
• Observed Molecular Weight: 24-28 kDa
• GenBank Accession Number: BC011960
• Gene Symbol: PSMD10
• Gene ID (NCBI): 5716
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O75832
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMD10, also known as Gankyrin, is a subunit of the 19S regulatory particle of the proteasome and plays a crucial role in protein degradation. It is a small, 25 kDa cytoplasmic-nuclear shuttling protein, highly conserved throughout evolution (~40% identity to yeast Nas6p). PSMD10 is involved in the regulation of cell cycle progression and apoptosis by interacting with key proteins such as p53 and Rb. PSMD10 has been identified as an oncoprotein, promoting tumor cell proliferation and survival in various cancers (PMID: 16581249, PMID: 31576540).