PSMD10/Gankyrin Recombinant antibody

PSMD10/Gankyrin Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 86120-2-RR
Clone No.250170D7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

PSMD10, 26S proteasome non-ATPase regulatory subunit 10, Gankyrin, p28, p28(GANK)

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, PC-3 cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86120-2-RR targets PSMD10/Gankyrin in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSMD10/Gankyrin fusion protein Ag2994 Predict reactive species
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 10
Calculated Molecular Weight 226 aa, 24 kDa
Observed Molecular Weight 24-28 kDa
GenBank Accession NumberBC011960
Gene Symbol PSMD10
Gene ID (NCBI) 5716
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75832
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMD10, also known as Gankyrin, is a subunit of the 19S regulatory particle of the proteasome and plays a crucial role in protein degradation. It is a small, 25 kDa cytoplasmic-nuclear shuttling protein, highly conserved throughout evolution (~40% identity to yeast Nas6p). PSMD10 is involved in the regulation of cell cycle progression and apoptosis by interacting with key proteins such as p53 and Rb. PSMD10 has been identified as an oncoprotein, promoting tumor cell proliferation and survival in various cancers (PMID: 16581249, PMID: 31576540).

Protocols

Product Specific Protocols
WB protocol for PSMD10/Gankyrin antibody 86120-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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