PSMD11 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86596-3-RR (same clone as 86596-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86596-3-RR (PSMD11 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 86596-3-RR (same clone as 86596-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 86596-3-RR (PSMD11 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 86596-3-RR (same clone as 86596-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMD11 antibody (86596-3-RR, Clone: 251499B5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86596-3-PBS in a different storage buffer formulation.

PSMD11 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86596-3-PBS

Clone No.251499B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

26S proteasome non-ATPase regulatory subunit 11, p44.5, Rpn6, S9

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Product Information

86596-3-PBS targets PSMD11 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6435

  Product name: Recombinant human PSMD11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 76-422 aa of BC000437

  Sequence: FLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYAGRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKKLT

• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC000437
• Gene Symbol: PSMD11
• Gene ID (NCBI): 5717
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00231
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.