PSMD12 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSMD12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11412-1-AP

Synonyms

p55, PSMD12, Rpn5

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Validation Data Gallery

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  WB analysis using 11412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11412-1-AP (PSMD12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 11412-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11412-1-AP (PSMD12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11412-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11412-1-AP (PSMD12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse lung tissue, rat lung tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11412-1-AP targets PSMD12 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMD12 fusion protein Ag1957
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
• Calculated Molecular Weight: 456 aa, 53 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC019062
• Gene Symbol: PSMD12
• Gene ID (NCBI): 5718
• RRID: AB_10640803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteasome 26S subunit, non-ATPase 12 (PSMD12; also known as RPN5) was first discovered in fission yeast. It is a non-ATPase subunit of the 19S regulator of the 26S proteasome complex, which is involved in regulating the cell cycle, repairing DNA damage, and regulating apoptosis by mediating the internal protein balance through the removal of misfolded proteins or damaged intracellular substructures. In addition, PSMD12 contributes to tumor cell growth and apoptosis in breast cancer. (PMID: 33987398)

Publications

Species	Application	Title
human	WB,IHC	Ann Transl Med; PSMD12 promotes glioma progression by upregulating the expression of Nrf2.; Authors - Zhongyong Wang ; KD Validated
human	WB	Biochem Biophys Res Commun; PSMD12 promotes breast cancer growth via inhibiting the expression of pro-apoptotic genes.; Authors - Xinna Du ; KD Validated