PSMD12 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86200-3-RR (same clone as 86200-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86200-3-RR (PSMD12 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86200-3-PBS in a different storage buffer formulation.

PSMD12 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86200-3-PBS

Clone No.250848B1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

26S proteasome non-ATPase regulatory subunit 12, p55, Rpn5, STISS

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Product Information

86200-3-PBS targets PSMD12 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1957

  Product name: Recombinant human PSMD12 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-320 aa of BC019062

  Sequence: MADGGSERADGRIVKMEVDYSATVDQRLPECAKLAKEGRLQEVIETLLSLEKQTRTASDMVSTSRILVAVVKMCYEAKEWDLLNENIMLLSKRRSQLKQAVAKMVQQCCTYVEEITDLPIKLRLIDTLRMVTEGKIYVEIERARLTKTLATIKEQNGDVKEAASILQELQVETYGSMEKKERVEFILEQMRLCLAVKDYIRTQIISKKINTKFFQEENTEKLKLKYYNLMIQLDQHEGSYLSICKHYRAIYDTPCIQAESEKWQQALKSVVLYVILAPFDNEQSDLVHRISGDKKLEEIPKYKDLLKLFTTMELMRWSTL

• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
• Calculated Molecular Weight: 456 aa, 53 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC019062
• Gene Symbol: PSMD12
• Gene ID (NCBI): 5718
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00232
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Proteasome 26S subunit, non-ATPase 12 (PSMD12; also known as RPN5) was first discovered in fission yeast. It is a non-ATPase subunit of the 19S regulator of the 26S proteasome complex, which is involved in regulating the cell cycle, repairing DNA damage, and regulating apoptosis by mediating the internal protein balance through the removal of misfolded proteins or damaged intracellular substructures. In addition, PSMD12 contributes to tumor cell growth and apoptosis in breast cancer. (PMID: 33987398)