PSMD5 Monoclonal antibody, PBS Only

PSMD5 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, pig, rat, rabbit, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D5F7

Cat no : 67682-1-PBS

Synonyms

26S protease subunit S5 basic, 26S proteasome subunit S5B, KIAA0072, PSMD5, S5B

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Validation Data Gallery

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  WB analysis of pig liver using 67682-1-Ig (same clone as 67682-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit liver using 67682-1-Ig (same clone as 67682-1-PBS)

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 67682-1-Ig (same clone as 67682-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67682-1-Ig (same clone as 67682-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HCT 116 using 67682-1-Ig (same clone as 67682-1-PBS)

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 67682-1-Ig (PSMD5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67682-1-Ig (same clone as 67682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67682-1-Ig (same clone as 67682-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67682-1-Ig (PSMD5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67682-1-Ig (same clone as 67682-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PSMD5 antibody (67682-1-Ig, Clone: 1D5F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 67682-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67682-1-PBS targets PSMD5 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rat, rabbit, mouse samples.

• Tested Reactivity: Human, pig, rat, rabbit, mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: PSMD5 fusion protein Ag19298
• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 5
• Calculated Molecular Weight: 504 aa, 56 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC014478
• Gene Symbol: PSMD5
• Gene ID (NCBI): 5711
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.