PSMD6 Polyclonal antibody

PSMD6 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12539-1-AP

Synonyms

KIAA0107, p42A, p44S10, PFAAP4, PSMD6, Rpn7, S10, SGA 113M



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse testis tissue, mouse brain tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12539-1-AP targets PSMD6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMD6 fusion protein Ag3251
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 6
Calculated molecular weight 389 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC012369
Gene symbol PSMD6
Gene ID (NCBI) 9861
RRIDAB_2170789
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD6 gene encodes 26S proteasome regulatory subunit RPN7, the characteristic of this protein is not well known to date.

Protocols

Product Specific Protocols
WB protocol for PSMD6 antibody 12539-1-APDownload protocol
IHC protocol for PSMD6 antibody 12539-1-APDownload protocol
IP protocol for PSMD6 antibody 12539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cells

The Identification of Nuclear FMRP Isoform Iso6 Partners

Authors - Nassim Ledoux