PSMD6 Polyclonal antibody
PSMD6 Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP,ELISA
Conjugate
Unconjugated
Cat no : 12539-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, HeLa cells, Jurkat cells |
Positive IP detected in | K-562 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Sample-dependent, check data in validation data gallery |
Product Information
12539-1-AP targets PSMD6 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PSMD6 fusion protein Ag3251 |
Full Name | proteasome (prosome, macropain) 26S subunit, non-ATPase, 6 |
Calculated molecular weight | 389 aa, 46 kDa |
Observed molecular weight | 46 kDa |
GenBank accession number | BC012369 |
Gene symbol | PSMD6 |
Gene ID (NCBI) | 9861 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD6 gene encodes 26S proteasome regulatory subunit RPN7, the characteristic of this protein is not well known to date.
Protocols
Product Specific Protocols | |
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WB protocol for PSMD6 antibody 12539-1-AP | Download protocol |
IP protocol for PSMD6 antibody 12539-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |