PSMD7 Monoclonal antibody, PBS Only

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  WB analysis using 68188-1-Ig (same clone as 68188-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68188-1-Ig (PSMD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68188-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 68188-1-Ig (same clone as 68188-1-PBS)

  WB result of PSMD7 antibody (68188-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD7 transfected HeLa cells. This data was developed using the same antibody clone with 68188-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

PSMD7 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 68188-1-PBS

Clone No.3E10E7

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, Indirect ELISA

Synonyms

MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12

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Product Information

68188-1-PBS targets PSMD7 in WB, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9305

  Product name: Recombinant human PSMD7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-319 aa of BC012606

  Sequence: MPELAVQKVVVHPLVLLSVVDHFNRIGKVGNQKRVVGVLLGSWQKKVLDVSNSFAVPFDEDDKDDSVWFLDHDYLENMYGMFKKVNARERIVGWYHTGPKLHKNDIAINELMKRYCPNSVLVIIDVKPKDLGLPTEAYISVEEVHDDGTPTSKTFEHVTSEIGAEEAEEVGVEHLLRDIKDTTVGTLSQRITNQVHGLKGLNSKLLDIRSYLEKVATGKLPINHQIIYQLQDVFNLLPDVSLQEFVKAFYLKTNDQMVVVYLASLIRSVVALHNLINNKIANRDAEKKEGQEKEESKKDRKEDKEKDKDKEKSDVKKEE

• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
• Calculated Molecular Weight: 324 aa, 37 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC012606
• Gene Symbol: PSMD7
• Gene ID (NCBI): 5713
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P51665
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)