PSME2 Recombinant monoclonal antibody

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• PSME2 Recombinant monoclonal antibody (87958-1-RR)

• 

  WB analysis using 87958-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87958-1-RR (PSME2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 87958-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 87958-1-RR (PSME2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 87958-1-RR

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 87958-1-RR (PSME2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity of 87958-1-RR

  Biolayer interferometry (BLI) kinetic assays of 87958-1-RR against Human PSME2 were performed. The affinity constant is 0.52 nM.

PSME2 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 87958-1-RR

Clone No.253556B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PA28B, PA28beta, REG beta, REGbeta, REG-beta

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, HepG2 cells, MCF-7 cells, 4T1 cells, Neuro-2a cells, PC-12 cells
• Positive IHC detected in: rat liver tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87958-1-RR targets PSME2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag3986

  Product name: Recombinant human PSME2 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-239 aa of BC019885

  Sequence: MAKPCGVRLSGEARKQVEVFRQNLFQEAEEFLYRFLPQKIIYLNQLLQEDSLNVADLTSLRAPLDIPIPDPPPKDDEMETDKQEKKEVPKCGFLPGNEKVLSLLALVKPEVWTLKEKCILVITWIQHLIPKIEDGNDFGVAIQEKVLERVNAVKTKVEAFQTTISKYFSERGDAVAKASKETHVMDYRALVHERDEAAYGELRAMVLDLRAFYAELYHIISSNLEKIVNPKGEEKPSMY

• Full Name: proteasome (prosome, macropain) activator subunit 2 (PA28 beta)
• Calculated Molecular Weight: 239 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC019885
• Gene Symbol: PSME2
• Gene ID (NCBI): 5721
• RRID: AB_3745837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UL46
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The principal function of the proteasome is targeted degradation of intracellular proteins. Activity of the 20S proteasome is controlled by regulatory complexes that bind to the ends of the cylindrical proteasome. 11S regulator (REG or PA28), is a complex of 28 kDa subunits that is thought to activate proteasomes toward the production of antigenic peptides. Human PSME1 and PSME2 genes encode the two proteasome activators PA28 alpha and beta, respectively, which have been implicated in antigen processing for loading class I MHC molecules. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.