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PSME4 Polyclonal antibody, PBS Only (18799-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18799-1-AP (PSME4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.
IP experiment of HEK-293 using 18799-1-AP (same clone as 18799-1-PBS)
IP result of anti-PSME4 (IP:18799-1-AP, 3ug; Detection:18799-1-AP 1:500) with HEK-293 cells lysate 1760ug. This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 18799-1-AP (same clone as 18799-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18799-1-AP (PSME4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)
Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 18799-1-AP (same clone as 18799-1-PBS)
Immunofluorescent analysis of Hela cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 18799-1-AP (same clone as 18799-1-PBS)
Immunofluorescent analysis of HepG2 cells, using PSME4 antibody 18799-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18799-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PSME4 Polyclonal antibody, PBS Only

Name: PSME4 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 18799-1-PBS

PSME4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 18799-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

18799-1-PBS targets PSME4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	proteasome (prosome, macropain) activator subunit 4
Calculated Molecular Weight	211 kDa
Observed Molecular Weight	140 kDa
GenBank Accession Number	BC113668
Gene Symbol	PSME4
Gene ID (NCBI)	23198
RRID	AB_10598020
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14997
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PSME4, like PSME1, 2, and 3, encodes proteasome activator complex subunit 4 also named proteasome activator 200 kDa (PA200). PA200 activates proteasomal cleavage of peptides in an energy-independent manner. After exposure of cells to ionizing radiation, PA200 forms nuclear foci and enhances proteasome activity independent of the stage of cell cycle arrest. PA200 also may be involved in spermatogenesis. The antibody is expected to recognize all the 4 isoforms of PSME4 but appears to mainly detect isoform 2 of 140-kDa.