PSMG2 Polyclonal antibody

PSMG2 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10959-1-AP

Synonyms

CLAST3, HCCA3, HsT1707, MDS003, PAC 2, PAC2, PSMG2, TNFSF5IP1



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10959-1-AP targets PSMG2 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMG2 fusion protein Ag1400
Full Name proteasome (prosome, macropain) assembly chaperone 2
Calculated molecular weight 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC013356
Gene symbol PSMG2
Gene ID (NCBI) 56984
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Proteasome assembly chaperone 2 (PSMG2), also named as Hepatocellular carcinoma-susceptibility protein 3, Tumor necrosis factor superfamily member 5-induced protein 1. It has a function as a chaperone protein which promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization.It is widely expressed with highest levels in lung, brain and colon,moderately expressed in muscle, stomach, spleen and heart and weakly expressed in small intestine, pancreas and liver,highly expressed in hepatocellular carcinomas with low levels in surrounding liver tissue. Molecular mass of PSMG2 is 29kD.

Protocols

Product Specific Protocols
WB protocol for PSMG2 antibody 10959-1-APDownload protocol
IHC protocol for PSMG2 antibody 10959-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols