PSPH Polyclonal antibody

• Featured Product
• KD/KO Validated

PSPH Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

28

Reviews (1)

Cat no : 14513-1-AP

Synonyms

PSPase, PSP, O-phosphoserine phosphohydrolase, L-3-phosphoserine phosphatase, EC:3.1.3.3

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Validation Data Gallery

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  WB analysis using 14513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14513-1-AP (PSPH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-87 MG using 14513-1-AP

  WB result of PSPH antibody (14513-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSPH transfected U-87 MG cells.

• 

  WB analysis of rat liver using 14513-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14513-1-AP (PSPH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 14513-1-AP

  IP result of anti-PSPH (IP:14513-1-AP, 4ug; Detection:14513-1-AP 1:300) with HL-60 cells lysate 3040ug.

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  IHC staining of human spleen using 14513-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14513-1-AP (PSPH Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14513-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14513-1-AP (PSPH Antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14513-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (14513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: A375 cells, rat liver tissue, U-87 MG cells, HL-60 cells, MCF-7 cells, SK-BR-3 cells
• Positive IP detected in: HL-60 cells
• Positive IHC detected in: human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 25 publications below
• IHC: See 7 publications below
• IP: See 1 publications below

Product Information

14513-1-AP targets PSPH in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSPH fusion protein Ag5972
• Full Name: phosphoserine phosphatase
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25-28 kDa
• GenBank Accession Number: BC063614
• Gene Symbol: PSPH
• Gene ID (NCBI): 5723
• RRID: AB_2171464
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).

Publications

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Cell Metab; Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.; Authors - Liang Wei Wang
human	WB	Cell Metab; Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation.; Authors - Long Shen
human	WB,IP	Nat Commun; PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma; Authors - Kui Wang
human	WB	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
human	WB	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Hua (Verified Customer) (11-30-2022)

Good antibody for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 cells, mouse liver, and mouse brain

PSPH Antibody Western Blot validation (1:1000 dilution) in HepG2 cells, mouse liver, and mouse brain (Cat no:14513-1-AP)