persephin-Specific Polyclonal antibody

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  WB analysis using 19713-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 19713-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19713-1-AP (persephin-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 19713-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary cancer using 19713-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 19713-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 19713-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 19713-1-AP (persephin-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 19713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 19713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19713-1-AP (persephin-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 19713-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using persephin-Specific antibody (19713-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of SH-SY5Y using 19713-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

• 

  FC experiment of HepG2 using 19713-1-AP

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug persephin-Specific Polyclonal antibody (19713-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

persephin-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 19713-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

persephin, PSPN, PSP

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, HepG2 cells
• Positive IHC detected in: human colon cancer tissue, human brain tissue, human ovary cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells
• Positive FC (Intra) detected in: SH-SY5Y cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

19713-1-AP targets persephin-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: persephin
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: NM_004158
• Gene Symbol: Persephin/PSPN
• Gene ID (NCBI): 5623
• RRID: AB_10640431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O60542
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSPN, also named as PSP, belongs to the TGF-beta family and GDNF subfamily. PSPN exhibits neurotrophic activity on mesencephalic dopaminergic and motor neurons. The antibody is specific to PSPN.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Biol Cell; Vps15p regulates the distribution of cup-shaped organelles containing the major eisosome protein Pil1p to the extracellular fraction required for endocytosis of extracellular vesicles carrying metabolic enzymes.; Authors - Kathryn Stein