PSRC1 Polyclonal antibody

PSRC1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27130-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

DDA3, FP3214, Proline/serine-rich coiled-coil protein 1, RP11 297O4.2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of A431 using 27130-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 27130-1-AP (PSRC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293T using 27130-1-AP
HEK-293T cells were subjected to SDS PAGE followed by western blot with 27130-1-AP (PSRC1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 27130-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27130-1-AP (PSRC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 27130-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27130-1-AP (PSRC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	A431 cells, HEK-293T cells
Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27130-1-AP targets PSRC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag26039 Product name: Recombinant human PSRC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-215 aa of BC056909 Sequence: MEDLEEDVRFIVDETLDFGGLSPSDSREEEDITVLVTPEKPLRRGLSHRSDPNAVAPAPQGVRLSLGPLSPEKLEEILDEANRLAAQLEQCALQDRESAGEGLGPRRVKPSPRRETFVLKDSPVRDLLPTVNSLTRSTPSPSSLTPRLRSNDRKGSVRALRATSGKRPSNMKRESPTCNLFPASKSPASSPLTRSTPPVRGRAGPSGRAAASEET Predict reactive species
Full Name	proline/serine-rich coiled-coil 1
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	39 kDa
GenBank Accession Number	BC056909
Gene Symbol	PSRC1
Gene ID (NCBI)	84722
RRID	AB_2880765
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6PGN9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for PSRC1 antibody 27130-1-AP	Download protocol
WB protocol for PSRC1 antibody 27130-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The PSRC1 Polyclonal antibody (Cat# 27130-1-AP) has 1 total citation. It appears in Molecular Cell (IF 16), in a study titled "LZTFL1 rewires NADPH-glutathione metabolism to amplify ferroptosis" (PMID 42551439). The research field associated with this citation is ferroptosis and redox/metabolic regulation of cell death.