PTBP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PTBP1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, RIP, IHC, ELISA

Conjugate

Unconjugated

Publications

40

Cat no : 12582-1-AP

Synonyms

HNRNP I, HNRNPI, HNRPI, pPTB, PTB, PTB 1, PTB T, PTB2, PTB3, PTB4, PTBP1

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Validation Data Gallery

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  WB analysis of HeLa using 12582-1-AP

  WB result of PTBP1 antibody (12582-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTBP1 transfected HeLa cells.

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  WB analysis using 12582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 12582-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 12582-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12582-1-AP (PTBP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12582-1-AP (PTBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.

• 

  IHC staining of human pancreas using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas slide using 12582-1-AP (PTBP1 Antibody) at dilution of 1:50.

• 

  IHC staining of mouse brain using 12582-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12582-1-AP (PTBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 12582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 12582-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12582-1-AP (PTBP1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, rat heart tissue, mouse lung tissue, BxPC-3 cells, Jurkat cells, K-562 cells
• Positive IHC detected in: human prostate cancer tissue, human pancreas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 8 publications below
• WB: See 33 publications below
• IHC: See 3 publications below
• IF: See 8 publications below
• IP: See 2 publications below
• RIP: See 4 publications below

Product Information

12582-1-AP targets PTBP1 in WB, IP, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTBP1 fusion protein Ag3277
• Full Name: polypyrimidine tract binding protein 1
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC013694
• Gene Symbol: PTBP1
• Gene ID (NCBI): 5725
• RRID: AB_2176402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10

Publications

Species	Application	Title
human	WB	Nat Cancer; SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers; Authors - Lei Shi
human	WB	Nat Cell Biol; Arg-tRNA synthetase links inflammatory metabolism to RNA splicing and nuclear trafficking via SRRM2; Authors - Haissi Cui
human	RIP	Blood; circMYBL2, a circRNA from MYBL2, regulates FLT3 translation by recruiting PTBP1 to promote FLT3-ITD AML progression.; Authors - Yu-Meng Sun
human	WB	Nat Commun; Systematic characterization of cancer transcriptome at transcript resolution; Authors - Wei Hu
rat	WB	Nucleic Acids Res; PMID: 30295850; Authors - Zonghong Li
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma