Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, BxPC-3 cells, K-562 cell |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55181-1-AP targets PTBP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | polypyrimidine tract binding protein 1 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 55-62 kDa |
| GenBank Accession Number | NM_002819 |
| Gene Symbol | PTBP1 |
| Gene ID (NCBI) | 5725 |
| RRID | AB_11182384 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26599 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Polypyrimidine tract-binding protein (PTBP1), also known as heterogeneous nuclear ribonucleoprotein type I (hnRNP I), is a nuclear protein that binds pre-mRNAs in specific regions of the hnRNA-protein complexes sensitive to micrococcal nuclease. It involves in pre-mRNA splicing and the regulation of alternative splicing events. PTBP1 promotes RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, and promotes the binding of U2 snRNP to pre-mRNA. Besides, it can cooperate with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA, and represses the splicing of MAPT/Tau exon 10
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTBP1 antibody 55181-1-AP | Download protocol |
| WB protocol for PTBP1 antibody 55181-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PTBP1 Polyclonal antibody (Cat# 55181-1-AP) has 1 citation. It was cited in Cell Research (2021) in a study comparing viral RNA-host protein interactomes across pathogenic RNA viruses to inform rapid antiviral drug discovery for SARS-CoV-2. The research field encompasses virology, host-pathogen interactions, and antiviral drug discovery. The antibody was used for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Cell Res Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2. |
