PTCD3 Polyclonal antibody

PTCD3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25158-1-AP

Synonyms

PTCD3, TRG 15

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Validation Data Gallery

• 

  WB analysis of mouse embryo using 25158-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 25158-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 25158-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25158-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 25158-1-AP

  IP result of anti-PTCD3 (IP:25158-1-AP, 4ug; Detection:25158-1-AP 1:600) with HEK-293 cells lysate 3600ug.

• 

  IF Staining of HEK-293 using 25158-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25158-1-AP (PTCD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse embryo tissue, HEK-293 cells, rat heart tissue, rat kidney tissue
• Positive IP detected in: HEK-293 cells
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

The immunogen of 25158-1-AP is PTCD3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTCD3 fusion protein Ag18330
• Full Name: Pentatricopeptide repeat domain 3
• Calculated Molecular Weight: 689 aa, 79 kDa
• Observed Molecular Weight: 78-80 kDa
• GenBank Accession Number: BC011832
• Gene Symbol: PTCD3
• Gene ID (NCBI): 55037
• RRID: AB_2879931
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
human	WB	Cell Death Dis; MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness.; Authors - Seongki Min
human	WB	Biomedicines; Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema; Authors - Loukmane Karim
human	WB	J Gen Virol; Inhibition of human cytomegalovirus replication by interferon alpha can involve multiple anti-viral factors; Authors - Shabab Chowdhury