|Positive WB detected in||HeLa cells, human colon tissue, human heart tissue, human kidney tissue, PC-3 cells|
|Positive IHC detected in||human lung cancer tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Sample-dependent, check data in validation data gallery|
10754-2-AP targets PTGDS in WB, IHC,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PTGDS fusion protein Ag1163|
|Full Name||prostaglandin D2 synthase 21kDa (brain)|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC005939|
|Gene ID (NCBI)||5730|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PGDS(Prostaglandin-H2 D-isomerase) is also named as PTGDS, PDS, PGDS2. It catalyzes the conversion of PGH2 to PGD2, and is a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. It has two N-glycosylation recognition sites.
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