CD315/PTGFRN Polyclonal antibody

• 

  WB analysis using 33863-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33863-1-AP (CD315/PTGFRN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 33863-1-AP

  IP result of anti-CD315/PTGFRN (IP:33863-1-AP, 4ug; Detection:33863-1-AP 1:300) with HeLa cells lysate 1480 ug.

CD315/PTGFRN Polyclonal Antibody for WB, IP, ELISA

Cat No. 33863-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

PTGFRN, CD315, EWIF, FPRP, KIAA1436

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse stomach tissue, rat stomach tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33863-1-AP targets CD315/PTGFRN in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg5010

  Product name: Recombinant human PTGFRN protein (C-rFc)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 26-832 aa of BC152454

  Sequence: VPTATLVRVVGTELVIPCNVSDYDGPSEQNFDWSFSSLGSSFVELASTWEVGFPAQLYQERLQRGEILLRRTANDAVELHIKNVQPSDQGHYKCSTPSTDATVQGNYEDTVQVKVLADSLHVGPSARPPPSLSLREGEPFELRCTAASASPLHTHLALLWEVHRGPARRSVLALTHEGRFHPGLGYEQRYHSGDVRLDTVGSDAYRLSVSRALSADQGSYRCIVSEWIAEQGNWQEIQEKAVEVATVVIQPSVLRAAVPKNVSVAEGKELDLTCNITTDRADDVRPEVTWSFSRMPDSTLPGSRVLARLDRDSLVHSSPHVALSHVDARSYHLLVRDVSKENSGYYYCHVSLWAPGHNRSWHKVAEAVSSPAGVGVTWLEPDYQVYLNASKVPGFADDPTELACRVVDTKSGEANVRFTVSWYYRMNRRSDNVVTSELLAVMDGDWTLKYGERSKQRAQDGDFIFSKEHTDTFNFRIQRTTEEDRGNYYCVVSAWTKQRNNSWVKSKDVFSKPVNIFWALEDSVLVVKARQPKPFFAAGNTFEMTCKVSSKNIKSPRYSVLIMAEKPVGDLSSPNETKYIISLDQDSVVKLENWTDASRVDGVVLEKVQEDEFRYRMYQTQVSDAGLYRCMVTAWSPVRGSLWREAATSLSNPIEIDFQTSGPIFNASVHSDTPSVIRGDLIKLFCIITVEGAALDPDDMAFDVSWFAVHSFGLDKAPVLLSSLDRKGIVTTSRRDWKSDLSLERVSVLEFLLQVHGSEDQDFGNYYCSVTPWVKSPTGSWQKEAEIHSKPVFITVKMDVLNAFKYP

• Full Name: prostaglandin F2 receptor negative regulator
• Calculated Molecular Weight: 879 aa, 99 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC152454
• Gene Symbol: PTGFRN
• Gene ID (NCBI): 5738
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9P2B2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGFRN (Prostaglandin F2 Receptor Negative Regulator), also known as CD315, is a trans-membrane protein that modulates prostaglandin F2α signaling and links the tetraspanin web to the cytoskeleton, it is now recognized as a driver of metastasis; high expression correlates with aggressive proliferation, migration, and spheroid growth in epidermoid carcinoma, medulloblastoma, and mesothelioma, while silencing PTGFRN impairs these traits and disrupts autophagy (PMID: 38924562). The calculated value of PTGFRN is 98 kDa. It has multiple glycosylation sites and its molecular weight after glycosylation is approximately 100 - 130 kDa.