PYK2 Polyclonal antibody

PYK2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 17592-1-AP


CADTK, CAK beta, CAKB, Cell adhesion kinase beta, FADK 2, FADK2, FAK2, Focal adhesion kinase 2, FRNK, PKB, Proline rich tyrosine kinase 2, Protein tyrosine kinase 2 beta, PTK, PTK2B, PYK2, RAFTK

Tested Applications

Positive WB detected inJurkat cells, rat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:3000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17592-1-AP is PYK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYK2 fusion protein Ag11487
Full Name PTK2B protein tyrosine kinase 2 beta
Calculated Molecular Weight 1009 aa, 116 kDa
Observed Molecular Weight 112-115 kDa
GenBank Accession NumberBC042599
Gene Symbol PTK2B
Gene ID (NCBI) 2185
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proline-rich tyrosine kinase 2 (Pyk2; also known as CAK, RAFTK and CADTK) is a cytoplasmic tyrosine kinase implicated to play a role in several intracellular signaling pathways. It is expressed in many cells and tissues and migrated as a 130 kDa band in Western Blotting analysis. The size of PYK2 is 115 kDa, and it expressed in many cells and tissues and migrated as a 130 kDa band, but several lower molecular weight bands (75 kDa, 80 kDa, and 97 kDa) were seen in Western Blotting analysis, possibly due to proteolytic degradation.


Product Specific Protocols
WB protocol for PYK2 antibody 17592-1-APDownload protocol
IHC protocol for PYK2 antibody 17592-1-APDownload protocol
IP protocol for PYK2 antibody 17592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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