PTK6/BRK Polyclonal antibody

• Featured Product
• KD/KO Validated

PTK6/BRK Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 18697-1-AP

Synonyms

PTK6, BRK, PTK6/protein tyrosine kinase 6, Protein-tyrosine kinase 6, Protein tyrosine kinase 6

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Validation Data Gallery

• 

  WB analysis of HeLa using 18697-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18697-1-AP (PTK6/BRK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 18697-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18697-1-AP (PTK6/BRK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18697-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18697-1-AP (PTK6/BRK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 18697-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 18697-1-AP (PTK6/BRK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 18697-1-AP

  IP result of anti-PTK6/BRK (IP:18697-1-AP, 4ug; Detection:18697-1-AP 1:800) with HeLa cells lysate 3440ug.

• 

  IHC staining of human colon using 18697-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18697-1-AP (PTK6/BRK antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 18697-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18697-1-AP (PTK6/BRK antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 18697-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PTK6/BRK antibody 18697-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, rat spleen tissue, mouse spleen tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

18697-1-AP targets PTK6/BRK in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PTK6 protein tyrosine kinase 6
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC035843
• Gene Symbol: PTK6
• Gene ID (NCBI): 5753
• RRID: AB_2284691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRK, also named as PTK6 and FLJ42088, is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. PTK6 can phosphorylate KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. Overexpression of PTK6 in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of PTK6 has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. This antibody is specific to PTK6.

Publications

Species	Application	Title
human	WB	Mol Cancer Res; RSK Promotes Prostate Cancer Progression in Bone through ING3, CKAP2 and PTK6-mediated Cell Survival.; Authors - Guoyu Yu ; KD Validated
human	WB	Chin J Cancer; The expression and prognostic value of protein tyrosine kinase 6 in early-stage cervical squamous cell cancer.; Authors - Xiao-Jing Wang
human	WB	Dose Response; Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.; Authors - Jianfa Yu
human	IF	Adv Sci (Weinh); ETV4 Mediated Tumor-Associated Neutrophil Infiltration Facilitates Lymphangiogenesis and Lymphatic Metastasis of Bladder Cancer; Authors - Qiang Zhang ; KD Validated