PTMS Polyclonal antibody

PTMS Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12106-1-AP

Synonyms

parathymosin, ParaT

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Validation Data Gallery

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  WB analysis of U-937 using 12106-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 12106-1-AP (PTMS antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 12106-1-AP

  Immunofluorescent analysis of A549 cells using 12106-1-AP (PTMS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: U-937 cells
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12106-1-AP targets PTMS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTMS fusion protein Ag2751
• Full Name: parathymosin
• Calculated Molecular Weight: 12 kDa
• Observed Molecular Weight: 10-12 kDa
• GenBank Accession Number: BC017025
• Gene Symbol: PTMS
• Gene ID (NCBI): 5763
• RRID: AB_10665368
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTMS, also named as Parathymosin, is a small nuclear protein that can physically interact with glucocorticoid receptors (GR) and histone H1, respectively, and can serve as a coactivator of GR (PMID: 16150697; PMID: 15716277). It is widely distributed in mammalian tissues. Catalog#12106-1-AP is a rabbit polyclonal antibody raised against the full-length of human PTMS. The MW of this protein is 12 kDa, and this antibody specially recognises the 12 kDa protein.

Publications

Species	Application	Title
human	WB	Oncogene; USP33 facilitates the ovarian cancer progression via deubiquitinating and stabilizing CBX2; Authors - Jiming Chen