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PTN Polyclonal antibody

PTN Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27117-1-AP

Synonyms

HARP, HB GAM, HBBM, HBGF 8, HBGF8, HBNF, HBNF 1, HBNF1, Heparin binding brain mitogen, NEGF1, OSF 1, Osteoblast specific factor 1, pleiotrophin, PTN



Tested Applications

Positive WB detected infetal human brain tissue, U-87 MG cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27117-1-AP targets PTN in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTN fusion protein Ag26004
Full Name pleiotrophin
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC005916
Gene Symbol PTN
Gene ID (NCBI) 5764
RRIDAB_2880763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pleiotrophin (PTN) is a multifunctional, cationic, glycosaminoglycan-binding cytokine and growth factor involved in numerous physiological and pathological processes, including tissue repair and inflammation-related diseases. PTN is highly upregulated in the brain in different disorders characterized by overt neuroinflammation such as neurodegenerative diseases, drug addiction, traumatic injury, and ischemia. PTN is important for CNS repair and for survival and differentiation of dopaminergic neurons.

Protocols

Product Specific Protocols
WB protocol for PTN antibody 27117-1-APDownload protocol
IHC protocol for PTN antibody 27117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

Sci Bull (Beijing)

Single-cell dynamics of liver development in postnatal pigs

Authors - Lin Rao
humanWB

Oncol Rep

Luteolin suppresses colorectal cancer cell metastasis via regulation of the miR‑384/pleiotrophin axis.

Authors - Yuanyuan Yao
humanWB

Oral Dis

PTN inhibited chondrogenic differentiation potential of dental pulp stem cells

Authors - Chang Liu
IF

Cell Rep

Dissecting the spermatogonial stem cell niche using spatial transcriptomics

Authors - Shreya Rajachandran