PTP4A1/PRL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PTP4A1/PRL1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 11508-1-AP

Synonyms

HH72, PRL 1, PRL1, PTP(CAAX1), PTP(CAAXI), PTP4A1, PTP4A1/PRL1, PTPCAAX1

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Validation Data Gallery

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  WB analysis of HepG2 using 11508-1-AP

  WB result of PTP4A1/PRL1 antibody (11508-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP4A1/PRL1 transfected HepG2 cells.

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  WB analysis of HeLa using 11508-1-AP

  WB result of PTP4A1/PRL1 antibody (11508-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP4A1/PRL1 transfected HeLa cells.

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  WB analysis using 11508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11508-1-AP (PTP4A1/PRL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11508-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11508-1-AP (PTP4A1/PRL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11508-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11508-1-AP (PTP4A1/PRL1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11508-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11508-1-AP (PTP4A1/PRL1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Product Information

11508-1-AP targets PTP4A1/PRL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTP4A1/PRL1 fusion protein Ag2067
• Full Name: protein tyrosine phosphatase type IVA, member 1
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: BC023975
• Gene Symbol: PTP4A1
• Gene ID (NCBI): 7803
• RRID: AB_2237951
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).

Publications

Species	Application	Title
mouse	WB	Sci Signal; The adaptor SH2B1 and the phosphatase PTP4A1 regulate the phosphorylation of cytohesin-2 in myelinating Schwann cells in mice.; Authors - Yuki Miyamoto
human	WB	Oncotarget; Oncogenic ALK regulates EMT in non-small cell lung carcinoma through repression of the epithelial splicing regulatory protein 1.; Authors - Claudia Voena
human	WB,IHC	Int J Oncol; MicroRNA-1271 functions as a metastasis and epithelial-mesenchymal transition inhibitor in human HCC by targeting the PTP4A1/c-Src axis.; Authors - Chao Li ; KD Validated
human	WB	PLoS One; MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.; Authors - Zhou Chang C
human	WB	Oncol Lett; miR-339-3p inhibits proliferation and metastasis of colorectal cancer.; Authors - Chang Zhou
human	WB,IHC	Int J Cancer; ERα inhibits mesenchymal and amoeboidal movement of liver cancer cell via Gα12; Authors - Jessica Yun ; KD Validated