Tested Applications
| Positive WB detected in | LNCaP cells, HepG2 cells, HuH-7 cells, HT-29 cells, HEK-293 cells, K-562 cells |
| Positive IHC detected in | human appendicitis tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:238-1:952 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68582-1-Ig targets PTPIP51 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15135 Product name: Recombinant human PTPIP51 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-141 aa of BC008970 Sequence: ESDNERDSDKESEDGEDEVSCETVKMGRKDSLDLEEEAASGASSALEAGGSSGLEDVLPLLQQADELHRGDEQGKREGFQLLLNNKLVYGSRQDFLWRLARAYSDMCELTEEVSEKKSYALDGKEEAEAALEKGDESADCH Predict reactive species |
| Full Name | family with sequence similarity 82, member A2 |
| Calculated Molecular Weight | 470 aa, 52 kDa |
| Observed Molecular Weight | 52-60 kDa |
| GenBank Accession Number | BC008970 |
| Gene Symbol | PTPIP51 |
| Gene ID (NCBI) | 55177 |
| RRID | AB_3085282 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96TC7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618).The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTPIP51 antibody 68582-1-Ig | Download protocol |
| IHC protocol for PTPIP51 antibody 68582-1-Ig | Download protocol |
| WB protocol for PTPIP51 antibody 68582-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PTPIP51 Monoclonal antibody (1G11E2), catalog number 68582-1-Ig, has a total of 1 citation. This citation was published in Cell Reports (2025) by Rhoads et al., titled "Neurons and astrocytes have distinct organelle signatures and responses to stress." The research field encompasses neuroscience and cell biology, specifically investigating organelle dynamics and stress responses in neurons and astrocytes. The antibody was applied via Western blot (WB) using rat samples.
| Species | Application | Title |
|---|---|---|
Cell Rep Neurons and astrocytes have distinct organelle signatures and responses to stress. |
