Tested Applications
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11493-1-AP targets PTPMT1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2063 Product name: Recombinant human PTPMT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-201 aa of BC020242 Sequence: MAATALLEAGLARVLFYPTLLYTLFRGKVPGRAHRDWYHRIDPTVLLGALPLRSLTRQLVQDENVRGVITMNEEYETRFLCNSSQEWKRLGVEQLRLSTVDMTGIPTLDNLQKGVQFALKYQSLGQCVYVHCKAGRSRSATMVAAYLIQVHKWSPEEAVRAIAKIRSYIHIRPGQLDVLKEFHKQITARATKDGTFVISKT Predict reactive species |
| Full Name | protein tyrosine phosphatase, mitochondrial 1 |
| Calculated Molecular Weight | 201 aa, 23 kDa |
| Observed Molecular Weight | 21-23 kDa |
| GenBank Accession Number | BC020242 |
| Gene Symbol | PTPMT1 |
| Gene ID (NCBI) | 114971 |
| RRID | AB_2237967 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WUK0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein Tyrosine Phosphatase localized to the Mitochondrial 1 (PTPMT1) is a highly conserved, dual-specific phosphatase that resides on the matrix-facing side of the inner mitochondrial membrane (IMM). PTPMT1 is a mitochondrial phosphatase and a vital regulator of mitochondrial activities. Current work points to roles for PTPMT1 in the regulation of mitochondrial respiration, dynamics, and ultrastructure through the modulation of pyruvate importation, the tuning of TCA cycle activity, and the synthesis of cardiolipin. PTPMT1 is highly expressed in pancreatic b cells, whose mitochondria have the important function of coupling glucose metabolism to the secretion of insulin. (PMID: 16337125, PMID: 16061174, PMID: 33335799)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PTPMT1 antibody 11493-1-AP | Download protocol |
| WB protocol for PTPMT1 antibody 11493-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PTPMT1 Polyclonal antibody (Cat# 11493-1-AP) has 9 citations across journals including Autophagy, Advanced Science, Cell Death & Disease, Journal for Immunotherapy of Cancer, Free Radical Biology and Medicine, Cell Reports, American Journal of Physiology-Cell Physiology, and Molecular Cell Biology. Research fields span mitophagy, oncogenesis, cell death signaling, mitochondrial oxidative phosphorylation, disulfidptosis, leukemia, cardiolipin synthesis, necroptosis, and embryonic stem cell differentiation.
| Species | Application | Title |
|---|---|---|
Autophagy PRKAB2 as a tumor suppressor in renal cell carcinoma: inhibiting mitophagy via the LRPPRC-PRKN/parkin interaction and cardiolipin biosynthesis. | ||
Adv Sci (Weinh) Phosphorylated SHMT2 Regulates Oncogenesis Through m6 A Modification in Lung Adenocarcinoma | ||
Cell Death Dis 4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer | ||
J Immunother Cancer Antagonism of estrogen-related receptor-α inhibits mitochondrial oxidative phosphorylation and reduces M2 macrophage infiltration in endometrial cancer | ||
Free Radic Biol Med Alexidine dihydrochloride enhances the sensitivity of human hepatocellular carcinoma to disulfidptosis via ATF4-DDIT3 activation
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Cell Rep A generalized strategy to kill leukemic cells by targeting the regulatory systems governing mitochondrial membrane potential.
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Reviews
What customers say
The single review reports successful use of this antibody for Western Blot, confirming it detects PTPMT1 in mouse heart samples. The reviewer rated it 4 out of 5 stars. Overall, the feedback is positive, with the antibody performing as expected for its intended application in mouse tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tongbin (Verified Customer) (08-25-2020) | This antibody detects PTPMT1 in mouse heart samples.
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