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PTPN2 Polyclonal antibody, PBS Only (11214-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of mouse thymus using 11214-1-AP (same clone as 11214-1-PBS)
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 11214-1-AP (same clone as 11214-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
WB analysis of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)
HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 11214-1-AP (same clone as 11214-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
WB analysis of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)
HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
WB analysis of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)
HL-60 cells were subjected to SDS PAGE followed by western blot with 11214-1-AP (PTPN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
IP experiment of HL-60 using 11214-1-AP (same clone as 11214-1-PBS)
IP result of anti-PTPN2 (IP:11214-1-AP, 4ug; Detection:11214-1-AP 1:500) with HL-60 cells lysate 4000ug. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11214-1-AP (same clone as 11214-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11214-1-AP (PTPN2 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11214-1-AP (same clone as 11214-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 11214-1-AP (same clone as 11214-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11214-1-AP (PTPN2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 11214-1-AP (same clone as 11214-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PTPN2 antibody (11214-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11214-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PTPN2 Polyclonal antibody, PBS Only

Name: PTPN2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11214-1-PBS

PTPN2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11214-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

T-cell protein-tyrosine phosphatase, TC PTP, PTPT, PTPN 2, PTN2

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11214-1-PBS targets PTPN2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1709 Product name: Recombinant human PTPN2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-346 aa of BC016727 Sequence: MPTTIEREFEELDTQRRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQNAENDYINASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRIVEKESVKCAQYWPTDDQEMLFKETGFSVKLLSEDVKSYYTVHLLQLENINSGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGSLNPDHGPAVIHCSAGIGRSGTFSLVDTCLVLMEKGDDINIKQVLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKCIKGDSSIQKRWKELSKEDLSPAFDHSPNKIMTEKYNGNRIGLEEEKLTGDRCTGLSSKMQDTMEENSE Predict reactive species
Full Name	protein tyrosine phosphatase, non-receptor type 2
Calculated Molecular Weight	49 kDa
Observed Molecular Weight	45-50 kDa
GenBank Accession Number	BC016727
Gene Symbol	PTPN2
Gene ID (NCBI)	5771
RRID	AB_2173235
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P17706
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.