PTPN2 Monoclonal antibody, PBS Only

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  WB analysis using 67388-1-Ig (same clone as 67388-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67388-1-Ig (same clone as 67388-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 67388-1-Ig (same clone as 67388-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 67388-1-Ig (PTPN2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67388-1-Ig (same clone as 67388-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 67388-1-Ig (same clone as 67388-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67388-1-Ig (PTPN2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67388-1-PBS in a different storage buffer formulation.

PTPN2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67388-1-PBS

Clone No.2A1D1

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

2A1D1, EC:3.1.3.48, PTN2, PTPN 2, PTPT

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Product Information

67388-1-PBS targets PTPN2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29749

  Product name: Recombinant human PTPN2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-346 aa of BC016727

  Sequence: MPTTIEREFEELDTQRRWQPLYLEIRNESHDYPHRVAKFPENRNRNRYRDVSPYDHSRVKLQNAENDYINASLVDIEEAQRSYILTQGPLPNTCCHFWLMVWQQKTKAVVMLNRIVEKESVKCAQYWPTDDQEMLFKETGFSVKLLSEDVKSYYTVHLLQLENINSGETRTISHFHYTTWPDFGVPESPASFLNFLFKVRESGSLNPDHGPAVIHCSAGIGRSGTFSLVDTCLVLMEKGDDINIKQVLLNMRKYRMGLIQTPDQLRFSYMAIIEGAKCIKGDSSIQKRWKELSKEDLSPAFDHSPNKIMTEKYNGNRIGLEEEKLTGDRCTGLSSKMQDTMEENSE

• Full Name: protein tyrosine phosphatase, non-receptor type 2
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC016727
• Gene Symbol: PTPN2
• Gene ID (NCBI): 5771
• RRID: AB_2882632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P17706
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PTPN2(Tyrosine-protein phosphatase non-receptor type 2) is also named as PTPT and belongs to the protein-tyrosine phosphatase family. It may modulate beta-cell apoptosis and/or local release of inflammatory mediators in the course of a viral infection by acting, at least in part, at the pancreatic beta-cell level(PMID: 19825843). This protein has some isoforms produced by alternative splicing with the molecular mass of 40-50 kDa.