Various lysates were subjected to SDS PAGE followed by western blot with 19510-1-AP (PTPN20B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 19510-1-AP (PTPN20B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC staining of mouse tongue using 19510-1-AP
Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 19510-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using PTPN20 antibody (19510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed A431 cells using PTPN20 antibody (19510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
mouse tongue tissue, pig tongue tissue, rat tongue tissue
Positive IHC detected in
mouse tongue tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
A431 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
19510-1-AP targets PTPN20 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
PTPN20B, also named as PTPN20A, belongs to the protein-tyrosine phosphatase family and Non-receptor class subfamily. PTPN20B is a tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. PTPN20B has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates. PTPN20B catalyze the reaction: Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. The antibody can recognize the isoform 1,2,7,8,10,11,13,15.
Various lysates were subjected to SDS PAGE followed by western blot with 19510-1-AP (PTPN20B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse tongue using 19510-1-AP
Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 19510-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse spleen using 19510-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse tongue using 19510-1-AP
Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse tongue using 19510-1-AP
Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 19510-1-AP (PTPN20 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of A431 using 19510-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using PTPN20 antibody (19510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
At Proteintech, we pride ourselves on our antibody quality, customer service and transparency. As such, we are comparing our antibodies with other vendors, enabling easy identification and comparisons of key data to help you choose the suitable antibody for your needs.
We have selected the top cited antibodies from these vendors for you to compare.
