PTPN22 Recombinant antibody, PBS Only

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  IF Staining of Jurkat using 85062-4-RR (same clone as 85062-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PTPN22 antibody (85062-4-RR, Clone: 242688A2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85062-4-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 85062-4-RR (same clone as 85062-4-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PTPN22 Recombinant antibody (85062-4-RR, Clone:242688A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 85062-4-PBS in a different storage buffer formulation.

PTPN22 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Cat No. 85062-4-PBS

Clone No.242688A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

242688A2, EC:3.1.3.48, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, LyP, LYP1

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Product Information

85062-4-PBS targets PTPN22 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PTPN22 fusion protein Ag2397
• Full Name: protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
• Calculated Molecular Weight: 82 kDa, 92 kDa
• GenBank Accession Number: BC017785
• Gene Symbol: PTPN22
• Gene ID (NCBI): 26191
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y2R2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.