PTPN5 Polyclonal antibody

PTPN5 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14515-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

PTPN5, PTPSTEP, STEP

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIPIHC

WB analysis of HL-60 using 14515-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 14515-1-AP (PTPN5 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse brain using 14515-1-AP
IP result of anti-PTPN5 (IP:14515-1-AP, 4ug; Detection:14515-1-AP 1:1500) with mouse brain tissue lysate 1840 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse spleen using 14515-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse spleen using 14515-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 14515-1-AP (PTPN5 Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HL-60 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse spleen tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

14515-1-AP targets PTPN5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5974 Product name: Recombinant human PTPN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 217-565 aa of BC064807 Sequence: FDCVMDIKPEADPTSLTVKSMGLQERRGSNVSLTLDMCTPGCNEEGFGYLMSPREESAREYLLSASRVLQAEELHEKALDPFLLQAEFFEIPMNFVDPKEYDIPGLVRKNRYKTILPNPHSRVCLTSPDPDDPLSSYINANYIRGYGGEEKVYIATQGPIVSTVADFWRMVWQEHTPIIVMITNIEEMNEKCTEYWPEEQVAYDGVEITVQKVIHTEDYRLRLISLKSGTEERGLKHYWFTSWPDQKTPDRAPPLLHLVREVEEAAQQEGPHCAPIIVHCSAGIGRTGCFIATSICCQQLRQEGVVDILKTTCQLRQDRGGMIQTCEQYQFVHHVMSLYEKQLSHQSPE Predict reactive species
Full Name	protein tyrosine phosphatase, non-receptor type 5 (striatum-enriched)
Calculated Molecular Weight	64 kDa
Observed Molecular Weight	46-50 kDa, 61-66 kDa
GenBank Accession Number	BC064807
Gene Symbol	PTPN5
Gene ID (NCBI)	84867
RRID	AB_2173548
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P54829
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for PTPN5 antibody 14515-1-AP	Download protocol
IP protocol for PTPN5 antibody 14515-1-AP	Download protocol
WB protocol for PTPN5 antibody 14515-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
		J Comp Neurol Identification of hippocampal area CA2 in hamster and vole brain. Authors - Preston Nicole Siegler View Article
rat	WB	Mol Neurobiol The Weakened Interaction Between HECTD4 and GluN2B in Ischemic Stroke Promotes Calcium Overload and Brain Injury Through a Mechanism Involving the Decrease of GluN2B and MALT1 Ubiquitination Authors - Yi-Yue Zhang View Article