PTPN7 Polyclonal antibody

PTPN7 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15286-1-AP

Synonyms

BPTP 4, HEPTP, LC PTP, LPTP, PTPN7, PTPNI



Tested Applications

Positive WB detected inRaji cells, K-562 cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

15286-1-AP targets PTPN7 in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN7 fusion protein Ag7453
Full Name protein tyrosine phosphatase, non-receptor type 7
Calculated molecular weight 41 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC001746
Gene symbol PTPN7
Gene ID (NCBI) 5778
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTPN7, also known as haematopoietic PTP (HePTP), belongs to the R7 family of protein tyrosine phosphatases (PTPs) which all contain a conserved 16 amino acid sequence at their N-termini (PMID: 9857190). PTPN7, via negative regulation of MAPK signalling, plays a key role in T-cell and B-cell development and is the only known PTP whose expression in T-lymphocytes is induced by IL-2 (PMID: 15980098). PTPN7 is a 38 kDa non-receptor PTP expressed predominately in leukocytes, spleen and thymus (PMID: 1530918, 24265715, 19636879).

Protocols

Product Specific Protocols
WB protocol for PTPN7 antibody 15286-1-APDownload protocol
IHC protocol for PTPN7 antibody 15286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols