PTPRCAP / CD45AP Polyclonal antibody

PTPRCAP / CD45AP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 19715-1-AP

Synonyms

PTPRCAP, PTPRC associated protein, Protein tyrosine phosphatase receptor type C-associated protein, Lymphocyte phosphatase-associated phosphoprotein, LPAP

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Validation Data Gallery

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  WB analysis of Jurkat using 19715-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19715-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 19715-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 19715-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 19715-1-AP (PTPRCAP / CD45AP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Jurkat using 19715-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using PTPRCAP / CD45AP antibody (19715-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, human spleen tissue
• Positive IHC detected in: human tonsillitis tissue, human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19715-1-AP targets PTPRCAP / CD45AP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protein tyrosine phosphatase, receptor type, C-associated protein
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: NM_005608
• Gene Symbol: PTPRCAP
• Gene ID (NCBI): 5790
• RRID: AB_10860107
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRCAP, also named as LPAP and CD45-AP, interacts with CD45/PTPRC.