PTPRD Polyclonal antibody

PTPRD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27941-1-AP

Synonyms

HPTP, HPTP DELTA, HPTPD, PTPD, PTPRD, R PTP DELTA

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Validation Data Gallery

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  WB analysis using 27941-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27941-1-AP (PTPRD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 27941-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27941-1-AP (PTPRD antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 27941-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27941-1-AP (PTPRD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 27941-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27941-1-AP (PTPRD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HuH-7 cells, human placenta tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27941-1-AP targets PTPRD in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPRD fusion protein Ag27689
• Full Name: protein tyrosine phosphatase, receptor type, D
• Calculated Molecular Weight: 1912 aa, 215 kDa
• Observed Molecular Weight: 75 kDa, 175 kDa
• GenBank Accession Number: BC106714
• Gene Symbol: PTPRD
• Gene ID (NCBI): 5789
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.