IA-2/PTPRN Monoclonal antibody

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  WB analysis using 66045-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66045-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66045-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 66045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 66045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human brain using 66045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human brain using 66045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 66045-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66045-1-Ig (IA-2/PTPRN antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 66045-1-Ig

  Immunofluorescent analysis of HepG2 cells using 66045-1-Ig (IA-2/PTPRN antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG.

IA-2/PTPRN Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66045-1-Ig

Clone No.1D3F5

Host / Isotype

Mouse / IgG3

Reactivity

human, pig, rabbit

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

IA-2, PTPRN, 1D3F5, ICA 512, ICA512-cleaved cytosolic fragment

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Tested Applications

• Positive WB detected in: pig brain tissue, human brain tissue, rabbit brain tissue
• Positive IHC detected in: human pancreas cancer tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66045-1-Ig targets IA-2/PTPRN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.

• Tested Reactivity: human, pig, rabbit
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag17909

  Product name: Recombinant human PTPRN protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 510-811 aa of BC007713

  Sequence: WCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ

• Full Name: protein tyrosine phosphatase, receptor type, N
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 60-65 kDa, 106-115 kDa
• GenBank Accession Number: BC007713
• Gene Symbol: IA-2/PTPRN
• Gene ID (NCBI): 5798
• RRID: AB_11064603
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16849
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.