PTRF Recombinant monoclonal antibody, PBS Only

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• PTRF Recombinant monoclonal antibody, PBS Only (82697-1-PBS)

PTRF Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 82697-1-PBS Copied!

Clone No. 3E15

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Cavin 1, Cavin-1, FKSG13

• 

  WB analysis using 82697-1-RR (same clone as 82697-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat lung using 82697-1-RR (same clone as 82697-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 82697-1-RR (PTRF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 82697-1-RR (same clone as 82697-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82697-1-RR (PTRF antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 82697-1-RR (same clone as 82697-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (82697-1-RR, Clone: 3E15 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82697-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 82697-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82697-1-RR against Human PTRF were performed. The affinity constant is 0.303 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

82697-1-PBS targets PTRF in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13486

  Product name: Recombinant human PTRF protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-198 aa of BC008849

  Sequence: MEDAAALELSSDEAVEVEEVIEESRAERIKRSGLRRVDDFKKAFSKEKMEKTKVRTRENLEKTRLKTKENLEKTRHTLEKRMNKLGTRLVPAERREKLKTSRDKLRKSFTPDHVVYARSKTAVYKVPPFTFHVKKIREGQVEVLKATEMVEVGADDDEGGAERGEAGDLRRGSSPDVHALLEITEESDAVLVDKSDSD

• Full Name: polymerase I and transcript release factor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC008849
• Gene Symbol: PTRF
• Gene ID (NCBI): 284119
• RRID: AB_3086511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q6NZI2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.