PUF60 Monoclonal antibody

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  WB analysis using 68533-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 68533-1-Ig

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 68533-1-Ig (PUF60 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

PUF60 Monoclonal Antibody for WB, ELISA

Cat No. 68533-1-Ig

Clone No.1A3A1

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, ELISA

Synonyms

FBP interacting repressor, FIR, FLJ31379, PUF60, Ro binding protein 1, RoBP1, RoBPI, Siah binding protein 1, Siah BP1, SIAHBP1

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Tested Applications

• Positive WB detected in: MCF-7 cells, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

68533-1-Ig targets PUF60 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PUF60 fusion protein Ag33930
• Full Name: poly-U binding splicing factor 60KDa
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC008875
• Gene Symbol: PUF60
• Gene ID (NCBI): 22827
• RRID: AB_3085241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UHX1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.

Publications

Species	Application	Title
mouse	WB	Food Funct; Flaxseed oil attenuates PFOS-induced testicular damage by regulating RNA alternative splicing; Authors - Jingyi Ren