PURA Monoclonal antibody

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  WB analysis using 67305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 67305-1-Ig

  WB result of PURA antibody (67305-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PURA transfected HepG2 cells.

• 

  WB analysis using 67305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67305-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67305-1-Ig (PURA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

PURA Monoclonal Antibody for WB, ELISA

Cat No. 67305-1-Ig

Clone No.1C6G4

Publications(1)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IF, ChIP, RIP, ELISA

Synonyms

PUR ALPHA, PUR1, PURA, PURALPHA

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Tested Applications

• Positive WB detected in: HepG2 cells, pig brain tissue, rat brain tissue, mouse brain tissue, L02 cells, LNCaP cells, human placenta tissue, rat cerebellum tissue, mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

67305-1-Ig targets PURA in WB, IF, ChIP, RIP, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12164

  Product name: Recombinant human PURA protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-322 aa of BC036087

  Sequence: MADRDSGSEQGGAALGSGGSLGHPGSGSGSGGGGGGGGGGGGSGGGGGGAPGGLQHETQELASKRVDIRNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYLGDFIEHYAQLGPSQPPDLAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEEEPAELPEGTSLTVDNKRFFFDVGSNKYGVFMRVSEVKPTYRNSITVPYKVWAKFGHTFCKYSEEMKKIQEKQREKRAACEQLHQQQQQQQEETAAATLLLQGEEEGEED

• Full Name: purine-rich element binding protein A
• Calculated Molecular Weight: 322 aa, 35 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC036087
• Gene Symbol: PURA
• Gene ID (NCBI): 5813
• RRID: AB_2882567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q00577
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	RIP,IF,ChIP	Int J Biol Macromol; MTA1-DT promotes endometrial cancer growth by modulating G2/M-related gene transcription via PURα; Authors - Yiting Liu