Tested Applications
| Positive WB detected in | C2C12 cells, mouse liver tissue, HeLa cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18128-1-AP targets PURB in WB, IF, IP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12705 Product name: Recombinant human PURB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-312 aa of BC101735 Sequence: MADGDSGSERGGGGGPCGFQPASRGGGEQETQELASKRLDIQNKRFYLDVKQNAKGRFLKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKENQRGRFLRIRQTVNRGGGGFGAGPGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGGPGGGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYADEMKEIQERQRDKLYERRGGGSGGGEESEGEEVDED Predict reactive species |
| Full Name | purine-rich element binding protein B |
| Calculated Molecular Weight | 312 aa, 33 kDa |
| Observed Molecular Weight | 33-40 kDa |
| GenBank Accession Number | BC101735 |
| Gene Symbol | PURB |
| Gene ID (NCBI) | 5814 |
| RRID | AB_2878504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96QR8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for PURB antibody 18128-1-AP | Download protocol |
| WB protocol for PURB antibody 18128-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PURB Polyclonal antibody (Cat# 18128-1-AP) has 14 citations spanning journals including Nature Communications, Molecular Cell, Nature Structural & Molecular Biology, Cell Reports, Free Radical Biology & Medicine, Molecular Metabolism, Stem Cells, Stem Cell Reports, Journal of Biological Chemistry, Journal of Cell Physiology, International Journal of Molecular Sciences, Frontiers in Molecular Neuroscience, Journal of Nanobiotechnology, and Tissue & Cell. Research fields cover cancer biology, transcriptional regulation, stem cell biology, ferroptosis, virology, metabolic regulation, and neuroscience.
| Species | Application | Title |
|---|---|---|
Nat Commun MaTAR25 lncRNA regulates the Tensin1 gene to impact breast cancer progression. | ||
Mol Cell Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles | ||
J Nanobiotechnology Targeted Inhibition of P. gingivalis OMV-derived TsRNA by tetrahedral framework nucleic acids promotes periodontal regeneration. | ||
Nat Struct Mol Biol The PURB-HOTAIR complex regulates p53-dependent promoter-specific transcriptional activation | ||
Free Radic Biol Med ALKBH5 targets ACSL4 mRNA stability to modulate ferroptosis in hyperbilirubinemia-induced brain damage | ||
Cell Rep Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infection.
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