Poliovirus receptor Polyclonal antibody

Poliovirus receptor Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27486-1-AP

Synonyms

CD155, HVED, Necl 5, NECL5, Nectin like protein 5, Poliovirus receptor, PVR, PVS, TAGE4

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Validation Data Gallery

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  WB analysis of HT-1080 using 27486-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 27486-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 27486-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (Poliovirus receptor antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 27486-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (Poliovirus receptor antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 27486-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (Poliovirus receptor antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HT-1080 cells, HUVEC cells, A549 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

27486-1-AP targets Poliovirus receptor in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Poliovirus receptor fusion protein Ag26657
• Full Name: poliovirus receptor
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC015542
• Gene Symbol: PVR
• Gene ID (NCBI): 5817
• RRID: AB_2880884
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang