CD155/PVR Monoclonal antibody

CD155/PVR Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1F12A1

Cat no : 66913-1-Ig

Synonyms

CD155, FLJ25946, HVED, Necl 5, NECL5, Nectin like protein 5, poliovirus receptor, PVR, PVS, TAGE4

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Validation Data Gallery

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  WB analysis using 66913-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66913-1-Ig (CD155/PVR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of K-562 using 66913-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66913-1-Ig (CD155/PVR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U2OS cells, K-562 cells, HT-1080 cells, U-251 cells, A549 cells, HT-29 cells, HepG2 cells, HeLa cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• ELISA: See 1 publications below

Product Information

66913-1-Ig targets CD155/PVR in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD155/PVR fusion protein Ag28303
• Full Name: poliovirus receptor
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC015542
• Gene Symbol: CD155/PVR
• Gene ID (NCBI): 5817
• RRID: AB_2882240
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD155, also known as PVR, is a type I transmembrane glycoprotein in the immunoglobulin superfamily. It contains three extracellular immunoglobulin-like domains, D1-D3, of which D1 is recognized by the virus. Mature human CD155 consists of a 323 amino acid extracellular domain with one N-terminal V-type and two C2-type Ig-like domains, a 24 amino acid transmembrane segment, and a 50 amino acid cytoplasmic tail. CD155 is thought to play a role in adhesion by interaction with the ECM component vitronectin as well as a role in NK killing of tumor cells. CD155 binds to two receptors of NK cells, CD96 and CD226, and accumulates at cell-cell contact sites, leading to the formation of mature immune synapses between NK cells and target cells. CD155 serves as the entry receptor for poliovirus and thereby mediates human susceptibility to poliovirus infection.

Publications

Species	Application	Title
human	ELISA	iScience; Urinary extracellular vesicles signature for diagnosis of kidney disease; Authors - Keiichi Takizawa