Nectin 2 Polyclonal antibody

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  WB analysis of mouse kidney using 10100-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10100-2-AP (Nectin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 10100-2-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10100-2-AP (Nectin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10100-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10100-2-AP (Nectin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Nectin 2 Polyclonal Antibody for WB, ELISA

Cat No. 10100-2-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: mouse kidney tissue, 3T3-L1 cells, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

10100-2-AP targets Nectin 2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nectin 2 fusion protein Ag0149
• Full Name: poliovirus receptor-related 2 (herpesvirus entry mediator B)
• Calculated Molecular Weight: 58 kDa
• GenBank Accession Number: BC003091
• Gene Symbol: Nectin-2/CD112
• Gene ID (NCBI): 5819
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92692
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Poliovirus receptor-related 2 (herpesvirus entry mediator B) (PVRL2, synonyms: HVEB, PRR2, CD112, PVRR2) is an adhesion molecule widely expressed in cell lines of different lineages, including hematopoietic, neuronal, endothelial and epithelial cells. CD112 belongs to a new family of immunoglobulin-like molecules that includes four members (CD111 , CD112, PRR3 and CD155) sharing an ectodomain made of three Ig domains, of V, C, C types. CD112 is expressed in the myelo-monocytic and megakaryocytic hematopoietic lineages and the function in hematopoiesis is currently unknown. CD112 is an intercellular homophilic adhesion. CD112 localizes specifically at adherents junctions via its cytoplasmic interaction with the scaffold F-actin binding protein afadin. This interaction is mediated by a sequence located to the C terminal ends of both isoforms of CD112 (A/ExYV) and the PDZ domain of afadin. This sequence is also found in CD112 and PRR3 and represents a specific consensus in the family, which is also named nectin family. Disruption of the murine CD112 gene leads to infertility of male mice with morphologically aberrant spermatozoa. CD112 mediates entry of some alphaherpesvirus mutants (also named HveB) via its V domain. CD112 is involved in cell to cell spreading of viruses

Publications

Species	Application	Title
monkey	WB,IF	Eur J Cell Biol; Membrane palmitoylated proteins regulate trafficking and processing of nectins.; Authors - Dudak Amanda A