Nectin 3 Recombinant monoclonal antibody, PBS Only (Capture)

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  WB analysis using 85833-4-RR (same clone as 85833-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85833-4-RR (PVRL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85833-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02144-3

  Sandwich ELISA standard curve of MP02144-3, Human Nectin 3 Recombinant Matched Antibody Pair - PBS only. 85833-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2657. 85833-3-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 ng/mL

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  Sample test of MP02144-3

  The mean Nectin 3 concentration was determined to be 269.06 ng/mL in MDA-MB-231 cell extract based on a 1.6 mg/mL extract load and 377.13 ng/mL in HCT116 cell extract based on a 1.3 mg/mL extract load.

Nectin 3 Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85833-4-PBS

Clone No.242804D5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Nectin-3, PVRL3, CD113, Nectin cell adhesion molecule 3, poliovirus receptor related 3

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Product Information

85833-4-PBS targets Nectin 3 as part of a matched antibody pair:

MP02144-3: 85833-4-PBS capture and 85833-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2657

  Product name: Recombinant Human Nectin 3 protein (rFc Tag) (HPLC verified)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 58-400 aa of NM_015480.3

  Sequence: GPIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQKVIYISDPPTTTTLQPTIQWHPSTADIEDLATEPKKLPFPLSTLATIKDD

• Full Name: poliovirus receptor-related 3
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: NM_015480.3
• Gene Symbol: Nectin-3
• Gene ID (NCBI): 25945
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQS3-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Nectin 3, also known as CD113, is a member of the nectin family of cell adhesion molecules. It plays a crucial role in cell-cell adhesion and the formation of adherens junctions, which are essential for maintaining tissue integrity and cell polarity. Nectin 3 can form homophilic and heterophilic interactions, meaning it can bind to itself or other nectin family members, such as Nectin-2 (PMID: 10744716; 22902367). These interactions are important for processes like cell migration, tissue development, and immune cell transmigration (PMID: 12901789; 24116228).