PXK Polyclonal antibody

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  WB analysis using 16315-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16315-1-AP (PXK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PXK Polyclonal Antibody for WB, ELISA

Cat No. 16315-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PX domain-containing protein kinase-like protein, MONaKA, Modulator of Na,K-ATPase, Modulator of Na,K ATPase

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16315-1-AP targets PXK in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PXK fusion protein Ag9362
• Full Name: PX domain containing serine/threonine kinase
• Calculated Molecular Weight: 450aa,52 kDa; 578aa,65 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC014479
• Gene Symbol: PXK
• Gene ID (NCBI): 54899
• RRID: AB_3669237
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z7A4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PXK is part of the sorting nexin (SNX) family of proteins, which are important for receptor internalization, organelle trafficking including endosomal trafficking, and other membrane-centric sorting functions. This is accomplished primarily through the PX-domain mediated binding of PI3P . PXK was first identified and cloned by two independent groups in 2005. Initial studies established that PXK is detectable in most tissues with a primarily cytoplasmic distribution. (PMID: 25620976)