ASC/TMS1 Recombinant monoclonal antibody

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  WB analysis using 83858-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83858-3-RR (PYCARD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 83858-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 83858-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83858-3-RR (ASC/TMS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83858-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83858-3-RR against Mouse PYCARD were performed. The affinity constant is 3.58 nM.

ASC/TMS1 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 83858-3-RR

Clone No.240908D5

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF, ELISA

Synonyms

Pycard, PYD and CARD domain containing, masc, CARD5, Asc

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Tested Applications

• Positive WB detected in: J774A.1 cells, mouse lung tissue, mouse thymus tissue, mouse spleen tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IF: See 1 publications below

Product Information

83858-3-RR targets ASC/TMS1 in WB, IHC, IF, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag32519

  Product name: Recombinant mouse PYCARD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-193 aa of NM_023258

  Sequence: MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS

• Full Name: PYD and CARD domain containing
• Calculated Molecular Weight: 21KD
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: NM_023258
• Gene Symbol: Pycard
• Gene ID (NCBI): 66824
• RRID: AB_3671442
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9EPB4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Diabetol Metab Syndr; Irisin regulates integrin αvβ5/FAK/ERK to inhibit neutrophil extracellular traps formation and reduce pancreatic beta-cells pyroptosis in type 2 diabetes mellitus; Authors - Anjun Tan
mouse	WB	Biochim Biophys Acta Mol Cell Res; Basic leucine zipper ATF-like transcription factor (Batf) aggravates central sensitization in nitroglycerin-induced chronic migraine mouse model.; Authors - Yanhong Liu
mouse	WB	Glia; WWP2 Overexpression Represses NLRP3 Inflammasome Activation in Cerebral Ischemia/Reperfusion Injury Through the Degradation of MAVS.; Authors - Hang Yu
	WB	Cytokine; Blocking Fpr3 ameliorates osteoarthritis by inhibiting NLRP3-mediated chondrocyte pyroptosis; Authors - Bin Zhou
mouse	WB	Cell Mol Life Sci; The E3 ubiquitin ligase MARCH9 alleviates pyroptosis by regulating NLPR3 ubiquitination following myocardial ischemia reperfusion.; Authors - Hongquan Lu
mouse	WB, IF	Adv Sci (Weinh); Targeting Microglial CD49a Inhibits Neuroinflammation and Demonstrates Therapeutic Potential for Parkinson's Disease.; Authors - Huanpeng Lu