PYCR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PYCR1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

52

Reviews (2)

Cat no : 13108-1-AP

Synonyms

PIG45, P5CR 1, P5CR, P5C reductase 1, P5C

---

Validation Data Gallery

• 

  WB analysis using 13108-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa cells using 13108-1-AP

  WB result of PYCR1 antibody (13108-1-AP, 1:5000) with si-Control and si-PYCR1 transfected HeLa cells.

• 

  WB analysis of mouse liver using 13108-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13108-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse embryo using 13108-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13108-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13108-1-AP (PYCR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13108-1-AP

  IP result of anti-PYCR1 (IP:13108-1-AP, 3ug; Detection:13108-1-AP 1:700) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human prostate cancer using 13108-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13108-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13108-1-AP (PYCR1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13108-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 13108-1-AP (PYCR1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 13108-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PYCR1 antibody (13108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of MCF-7 using 13108-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PYCR1 antibody (13108-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, human brain tissue, mouse embryo tissue, mouse liver tissue, NIH/3T3 cells, HT-1080 cells，mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 45 publications below
• IHC: See 12 publications below
• IF: See 12 publications below
• IP: See 7 publications below
• CoIP: See 1 publications below

Product Information

13108-1-AP targets PYCR1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PYCR1 fusion protein Ag3764
• Full Name: pyrroline-5-carboxylate reductase 1
• Calculated Molecular Weight: 319 aa, 33.8 kDa
• Observed Molecular Weight: 33 kDa, 35 kDa
• GenBank Accession Number: BC022244
• Gene Symbol: PYCR1
• Gene ID (NCBI): 5831
• RRID: AB_2174878
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35 kDa. PYCR1 has 3 isoforms produced by alternative splicing. This antibody may have cross reaction to PYCR2 due to the high homology.

Publications

Species	Application	Title
human	WB,IHC	Nature; Tumour-specific proline vulnerability uncovered by differential ribosome codon reading.; Authors - Fabricio Loayza-Puch ; KO Validated
human	WB	Nature; Functional genomics reveal that the serine synthesis pathway is essential in breast cancer.; Authors - Possemato Richard R
zebrafish	WB,IF	Nat Genet; Mutations in PYCR1 cause cutis laxa with progeroid features.; Authors - Reversade Bruno B
mouse	WB	Cell Metab; Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.; Authors - Sukru Anil Dogan
human	IHC	J Hepatol; Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis.; Authors - Zhaobing Ding
human,mouse	WB,IF	Neuron; Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.; Authors - Nathalie Escande-Beillard

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Hyeonju (Verified Customer) (05-13-2024)

Too many non-specific bands are captured. ? I'm curious what the band is under pycr1 in lanes 6-9. Can you send me information about other proteins that react with the pycr1 antibody(if you have information about this)

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver tissue

PYCR1 Antibody Western Blot validation (1:1000 dilution) in mouse liver tissue (Cat no:13108-1-AP)

FH

Daniel (Verified Customer) (02-05-2020)

The antibody stained the glomerulus of the mouse kidney.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse kidney

PYCR1 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse kidney (Cat no:13108-1-AP)