PYCR1 Antibody

PYCR1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H2G1

Cat no : 66510-1-Ig

Synonyms

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1



Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

66510-1-Ig targets PYCR1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PYCR1 fusion protein Ag17919
Full Name pyrroline-5-carboxylate reductase 1
Calculated molecular weight 319aa,33.8 kDa
Observed molecular weight 33 kDa, 35 kDa
GenBank accession number BC022244
Gene symbol PYCR1
Gene ID (NCBI) 5831
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa.

Protocols

Product Specific Protocols
WB protocol for PYCR1 antibody 66510-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols