PYGB Polyclonal antibody

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  WB analysis using 12075-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12075-1-AP (PYGB antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12075-1-AP

  WB result of PYGB antibody (12075-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells.

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  WB analysis of human brain using 12075-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12075-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 12075-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12075-1-AP (PYGB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12075-1-AP

  IP result of anti-PYGB (IP:12075-1-AP, 4ug; Detection:12075-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of rat brain using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 12075-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12075-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

PYGB Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12075-1-AP

Publications(19)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

EC:2.4.1.1, GPBB, phosphorylase, glycogen; brain

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Tested Applications

• Positive WB detected in: MCF-7 cells, human brain tissue, rat brain tissue, C2C12 cells, fetal human brain tissue, mouse brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human stomach cancer tissue, human ovary tumor tissue, mouse heart tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IP: See 2 publications below

Product Information

12075-1-AP targets PYGB in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2732

  Product name: Recombinant human PYGB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 544-843 aa of BC017045

  Sequence: LKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD

• Full Name: phosphorylase, glycogen; brain
• Calculated Molecular Weight: 843 aa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC017045
• Gene Symbol: PYGB
• Gene ID (NCBI): 5834
• RRID: AB_2174885
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11216
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The three main subtypes of GP found in human tissues-brain-type glycogen phosphorylase (PYGB), liver-type glycogen phosphorylase (PYGL), and muscle-type glycogen phosphorylase (PYGM)-differ in function, structure, and tissue distribution. PYGB (Brain‑type glycogen phosphorylase) is a glycogen phosphorylase and is primarily localized in adult brain tissues and embryo liver tissues, whose function is to provide energy to organisms (PMID: 30106110). PYGB has been observed to be overexpressed in many types of cancer and tumor cell lines and is expected to be a novel target for the diagnosis and treatment of various cancers (PMID: 38334681).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Brain; C9orf72 expansion within astrocytes reduces metabolic flexibility in amyotrophic lateral sclerosis.; Authors - Scott P Allen
human	WB	Int J Biol Sci; PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer; Authors - Qian Ji
human	WB	Mol Cell Proteomics; Multi-omics integration reveals the landscape of pro-metastasis metabolism in hepatocellular carcinoma.; Authors - Yongmei Li
		iScience; Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia; Authors - Yu-Han H Hsu
human	WB	Front Cell Dev Biol; Glycogen Phosphorylase B Is Regulated by miR101-3p and Promotes Hepatocellular Carcinoma Tumorigenesis.; Authors - Guangying Cui
mouse	WB	J Cell Mol Med; PMID: 21251210; Authors - Xi Lei L

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (02-25-2020)

works fine

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer