PYGB Recombinant monoclonal antibody, PBS Only

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• PYGB Recombinant monoclonal antibody, PBS Only (86737-3-PBS)

PYGB Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86737-3-PBS

Clone No.251450C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

EC:2.4.1.1, GPBB, phosphorylase, glycogen; brain

• 

  WB analysis using 86737-3-RR (same clone as 86737-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86737-3-RR (PYGB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86737-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 86737-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86737-3-RR against Human PYGB were performed. The affinity constant is below 1 pM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

86737-3-PBS targets PYGB in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2732

  Product name: Recombinant human PYGB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 544-843 aa of BC017045

  Sequence: LKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD

• Full Name: phosphorylase, glycogen; brain
• Calculated Molecular Weight: 843 aa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC017045
• Gene Symbol: PYGB
• Gene ID (NCBI): 5834
• RRID: AB_3745093
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11216
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The three main subtypes of GP found in human tissues-brain-type glycogen phosphorylase (PYGB), liver-type glycogen phosphorylase (PYGL), and muscle-type glycogen phosphorylase (PYGM)-differ in function, structure, and tissue distribution. PYGB (Brain‑type glycogen phosphorylase) is a glycogen phosphorylase and is primarily localized in adult brain tissues and embryo liver tissues, whose function is to provide energy to organisms (PMID: 30106110). PYGB has been observed to be overexpressed in many types of cancer and tumor cell lines and is expected to be a novel target for the diagnosis and treatment of various cancers (PMID: 38334681).