PYGO1 Polyclonal antibody

PYGO1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 22799-1-AP

Synonyms

PYGO1, Pygopus homolog 1, pygopus homolog 1 (Drosophila)

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Validation Data Gallery

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  WB analysis of HeLa using 22799-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22799-1-AP (PYGO1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 22799-1-AP is PYGO1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PYGO1 fusion protein Ag18814
• Full Name: pygopus homolog 1 (Drosophila)
• Calculated Molecular Weight: 419 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC146572
• Gene Symbol: PYGO1
• Gene ID (NCBI): 26108
• RRID: AB_2879171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Am J Physiol Heart Circ Physiol; Pygo1 regulates pathological cardiac hypertrophy via a β-catenin-dependent mechanism.; Authors - Li Lin
human	WB	Hum Cell; Overexpression of PYGO1 promotes early cardiac lineage development in human umbilical cord mesenchymal stromal/stem cells by activating the Wnt/β-catenin pathway; Authors - Jie Shen