PYGO2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PYGO2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11555-1-AP

Synonyms

PYGO 2, PP7910

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Validation Data Gallery

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  WB analysis of MDA-MB-453s using 11555-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11555-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11555-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 11555-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 11555-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11555-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11555-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11555-1-AP (PYGO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MDA-MB-453s cells, C6 cells, HeLa cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

11555-1-AP targets PYGO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PYGO2 fusion protein Ag2111
• Full Name: pygopus homolog 2 (Drosophila)
• Calculated Molecular Weight: 406 aa, 41 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC032099
• Gene Symbol: PYGO2
• Gene ID (NCBI): 90780
• RRID: AB_2877776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYGO2, also named as Pygopus homolog 2, is a 406 animo acid protein, which contains 1 PHD-type zinc finger. PYGO2 is involved in signal transduction through the Wnt pathway and Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex. PYGO2 is in a high proportion of breast and epithelial ovarian malignant tumors and is required for the growth of several cell lines derived from these carcinomas.

Publications

Species	Application	Title
human	WB,IHC	J Cancer; Pygo2 as a novel biomarker in gastric cancer for monitoring drug resistance by upregulating MDR1.; Authors - Dongdong Zhang ; KD Validated
human	WB	Nat Commun; Structural basis of the interaction between BCL9-Pygo and LDB-SSBP complexes in assembling the Wnt enhanceosome; Authors - Hongyang Wang